EasyClus check & Info of last file ----- Processed at : 21-Jul-2023 12:02:31 ----- 1. The cytometric analysis is performed on machine IFREMER 2. The CytoUSB software used is CytoUSB Version: 5.11.1.25 3. The filename is FLR15 2023-04-19 07h59.cyz 4. The analysis is performed on 19-04-2023 time 08:00:58 5. The number of measured particles is 8615 6. The total concentration found (e.g. only SWS trigg.) is 28 particles/microliter 7. The concentration found (all used triggers) is 28 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 81 particles/microliter 9. The flush time found (between samples) is 60 seconds 10. The effective analysis volume is 307.911 microliter 11. The pumped volume is 379.8 microliter 12. The particle-rate is 59 particles passing the laser beam/second 13. The used sample flow rate is 2.1106 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 15 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 29.5 C 18. Temperature sensor Sheath is : 28.9 C 19. Temperature sensor Sheath is : 28.2 C 20. Temperature sensor System is : 26.0 C 21. Pressure sensor difference is : -270 mBar 22. Pressure sensor absolute is : 253 mBar 23. Sample pump 1: Last calibration of the sample pump was : 02-Févr-2023 24. Alignment check 1 by curvature detector is 0.75099 (1=perfect) 25. Alignment check 2 by curvature detector is 36% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 0 (0% of 8615 measured particles) 28. FWS: Maximum signal height found is 1013 mV 29. FWS: Nr of particles with signal height <10 mV is high (46 part. (1 %)) 30. SWS HS: Nr of saturated signals is 17 (0% of 8615 measured particles) 31. SWS HS: Maximum signal height found is 5000 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (56 part. (1 %)) 33. FL Red HS: Nr of saturated signals is 0 (0% of 8615 measured particles) 34. FL Red HS: Maximum signal height found is 3781 mV 35. FL Red HS: Nr of particles with signal height <10 mV is high (9 part. (0 %)) 36. FL Yellow HS: Nr of saturated signals is 0 (0% of 8615 measured particles) 37. FL Yellow HS: Maximum signal height found is 35 mV 38. FL Yellow HS: Nr of particles with signal height <10 mV is high (8612 part. (100 %)) 39. FL Orange HS: Nr of saturated signals is 0 (0% of 8615 measured particles) 40. FL Orange HS: Maximum signal height found is 321 mV 41. FL Orange HS: Nr of particles with signal height <10 mV is high (8598 part. (100 %)) 42. FL Green HS: Nr of saturated signals is 0 (0% of 8615 measured particles) 43. FL Green HS: Maximum signal height found is 101 mV 44. FL Green HS: Nr of particles with signal height <10 mV is high (8613 part. (100 %)) 45. FWS: Nr of block shaped signals is 15 (0.17411%) 46. SWS HS: Nr of block shaped signals is 13 (0.1509%) 47. FL Red HS: Nr of block shaped signals is 1 (0.011608%) 48. Suspect nr: 0=low, 10=high 49. Maximum FWS: suspect = 0 50. Maximum SWS HS: suspect = 1 51. Maximum FL Red HS: suspect = 0 52. Maximum FL Yellow HS: suspect = 0 53. Maximum FL Orange HS: suspect = 0 54. Maximum FL Green HS: suspect = 3 55. SWS: PMT level is 60 56. FL Red: PMT level is 85 57. FL Yellow: PMT level is 90 58. FL Orange: PMT level is 85 59. FL Green: PMT level is 85 -------------------------- Warnings info -------------------------- 1.01 Warning: The number of saved particles in this analysis is quite low and 1.01 Warning: you might miss (statistically) bigger particles in your sample 1.01.1 Solution - Increase number of measured particles. Increase setted time OR after setted number of analyzed particles (in CytoUSB) 1.03 Warning: Your preconcentration differs more than 25% from the during analysis concentration 1.03.1 Solution - Carry over between samples? > Flush longer 1.03.2 Solution - Better homogenize samples during subsampling by a gentle stirrer 1.03.3 Solution - Colonies-filaments of cells break during analysis > stir slower 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.23 Warning: FWS-L and -R might indicate an air bubble problem 36% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.26 Warning: FL Yellow HS: Biggest particles found is only 35 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Yellow HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (100 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.26 Warning: FL Orange HS: Biggest particles found is only 321 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Orange HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (100 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.26 Warning: FL Green HS: Biggest particles found is only 101 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Green HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Green HS : Nr of particles with signal height <10 mV is high (100 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Green HS: suspect = 3 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'