EasyClus check & Info of last file ----- Processed at : 14-Jan-2023 08:36:53 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.11.1.6 3. The filename is SCOPES_flr1_sws10_maxsws4_5ul_16min 2023-01-14 08h19.cyz 4. The analysis is performed on 14-01-2023 time 08:20:23 5. The number of measured particles is 76721 6. The total concentration found (e.g. only SWS trigg.) is 24 particles/microliter 7. The concentration found (all used triggers) is 14 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 14 particles/microliter 9. The flush time found (between samples) is 60 seconds 10. The effective analysis volume is 5459.4922 microliter 11. The pumped volume is 5472 microliter 12. The particle-rate is 134 particles passing the laser beam/second 13. The used sample flow rate is 5.7015 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 1 mV 16. Trigger 2: Total SWS at threshold level 10 17. Trigger 3: Maximum SWS at threshold level 4 18. Temperature sensor PMT is : 25.8 C 19. Temperature sensor Sheath is : 24.5 C 20. Temperature sensor Sheath is : 23.9 C 21. Temperature sensor System is : 21.2 C 22. Pressure sensor difference is : -302 mBar 23. Pressure sensor absolute is : 209 mBar 24. Sample pump 1: Last calibration of the sample pump was : 19-Oct-2022 25. Alignment check 1 by curvature detector is 0.98551 (1=perfect) 26. Alignment check 2 by curvature detector is 31% (100%=perfect) 27. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 28. FWS: Nr of saturated signals is 1 (0% of 76721 measured particles) 29. FWS: Maximum signal height found is 5120 mV 30. FWS: Nr of particles with signal height <10 mV is high (42243 part. (55 %)) 31. SWS HS: Nr of saturated signals is 16 (0% of 76721 measured particles) 32. SWS HS: Maximum signal height found is 7360 mV 33. SWS HS: Nr of particles with signal height <10 mV is high (7436 part. (10 %)) 34. FL Yellow HS: Nr of saturated signals is 0 (0% of 76721 measured particles) 35. FL Yellow HS: Maximum signal height found is 4000 mV 36. FL Yellow HS: Nr of particles with signal height <10 mV is high (58274 part. (76 %)) 37. FL Orange HS: Nr of saturated signals is 0 (0% of 76721 measured particles) 38. FL Orange HS: Maximum signal height found is 1176 mV 39. FL Orange HS: Nr of particles with signal height <10 mV is high (63568 part. (83 %)) 40. FL Red HS: Nr of saturated signals is 1 (0% of 76721 measured particles) 41. FL Red HS: Maximum signal height found is 5664 mV 42. FL Red HS: Nr of particles with signal height <10 mV is high (41755 part. (54 %)) 43. FWS: Nr of block shaped signals is 17 (0.022158%) 44. SWS HS: Nr of block shaped signals is 16 (0.020855%) 45. FL Yellow HS: Nr of block shaped signals is 1 (0.0013034%) 46. Suspect nr: 0=low, 10=high 47. Maximum FWS: suspect = 0 48. Maximum SWS HS: suspect = 0 49. Maximum FL Yellow HS: suspect = 0 50. Maximum FL Orange HS: suspect = 0 51. Maximum FL Red HS: suspect = 0 52. SWS: PMT level is 60 53. FL Yellow: PMT level is 90 54. Fl Orange: PMT level is 85 55. FL Red: PMT level is 85 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.23 Warning: FWS-L and -R might indicate an air bubble problem 31% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FWS : Nr of particles with signal height <10 mV is high (55 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (76 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (83 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (54 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)