EasyClus check & Info of last file ----- Processed at : 08-Jul-2024 20:22:51 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoBuoy instrument serial-nr is CS-2019-93 3. The CytoUSB software used is CytoUSB Version: 5.11.1.6 4. The filename is SeineMO_Pico_continu_flr3_5ul_8min 2024-05-07 19h09.cyz 5. The analysis is performed on 07-05-2024 time 19:10:14 6. The number of measured particles is 206807 7. The total concentration found (e.g. only SWS trigg.) is 86 particles/microliter 8. The concentration found (all used triggers) is 86 particles/microliter 9. The preconcentration (before analysis) found (all used triggers) is 86 particles/microliter 10. The flush time found (between samples) is 50 seconds 11. The effective analysis volume is 2413.2837 microliter 12. The pumped volume is 2419.2 microliter 13. The particle-rate is 432 particles passing the laser beam/second 14. The used sample flow rate is 5.0417 microliter/second 15. Trigger 1: FL Red at threshold level 3 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 29.6 C 18. Temperature sensor Sheath is : 28.2 C 19. Temperature sensor Laser is : 27.4 C 20. Temperature sensor System is : 24.5 C 21. Pressure sensor difference is : -392 mBar 22. Pressure sensor absolute is : 261 mBar 23. Sample pump 1: Last calibration of the sample pump was : 25-Mar-2024 24. Alignment check 1 by curvature detector is 0.7646 (1=perfect) 25. Alignment check 2 by curvature detector is 34.1% (>70%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 6 (0% of 206807 measured particles) 28. FWS: Maximum signal height found is 14848 mV 29. FWS: Nr of particles with signal height <10 mV is high (147933 part. (72 %)) 30. SWS HS: Nr of saturated signals is 4631 (0% of 206807 measured particles) 31. SWS HS: Maximum signal height found is 7488 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (109313 part. (53 %)) 33. FL Yellow HS: Nr of saturated signals is 1 (0% of 206807 measured particles) 34. FL Yellow HS: Maximum signal height found is 5504 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (204090 part. (99 %)) 36. FL Orange HS: Nr of saturated signals is 1 (0% of 206807 measured particles) 37. FL Orange HS: Maximum signal height found is 6784 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (204267 part. (99 %)) 39. FL Red HS: Nr of saturated signals is 80 (0% of 206807 measured particles) 40. FL Red HS: Maximum signal height found is 7488 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (146928 part. (71 %)) 42. SWS HS: Nr of block shaped signals is 3829 (1.8515%) 43. FWS: Nr of block shaped signals is 2055 (0.99368%) 44. FL Yellow HS: Nr of block shaped signals is 5 (0.0024177%) 45. FL Red HS: Nr of block shaped signals is 1 (0.00048354%) 46. Suspect nr: 0=low, 10=high 47. Maximum FWS: suspect = 0 48. Maximum SWS HS: suspect = 0 49. Maximum FL Yellow HS: suspect = 0 50. Maximum FL Orange HS: suspect = 0 51. Maximum FL Red HS: suspect = 0 52. SWS: PMT level is 60 53. FL Yellow: PMT level is 90 54. Fl Orange: PMT level is 85 55. FL Red: PMT level is 85 -------------------------- Warnings info -------------------------- 1.02 Warning: The number of saved particles in this analysis is quite high 1.02 Warning: Is it really necessary to store so many particles? E.g. data processing will take longer 1.02.1 Solution - Do not stop analysis after setted time, but after setted number of analyzed particles (in CytoUSB) 1.04 Warning: Your flush time (50 seconds) is quite small 1.04.1 Solution - Flush longer e.g. 80 sec. 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.23 Warning: Percentage ratio FWS-L/-R data within bandwidth is small which might indicate an alignment problem 34.1% (>70%=perfect) 1.23.1 Solution - Maybe an air bubble or blockage problem? Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FWS : Nr of particles with signal height <10 mV is high (72 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: SWS HS : Nr of particles with signal height <10 mV is high (53 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (99 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (99 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (71 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)