EasyClus check & Info of last file ----- Processed at : 03-Jun-2022 19:49:08 ----- 1. The cytometric analysis is performed on machine MIO - La Réunion 2. The CytoUSB software used is CytoUSB Version: 5.11.0.17 3. The filename is MAP-IO-Resilience_22-FLR5 2022-05-21 19h22.cyz 4. The analysis is performed on 21-05-2022 time 19:22:09 5. The number of measured particles is 16279 6. The total concentration found (e.g. only SWS trigg.) is 14 particles/microliter 7. The concentration found (all used triggers) is 14 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 14 particles/microliter 9. The flush time found (between samples) is 100 seconds 10. The effective analysis volume is 1165.2256 microliter 11. The pumped volume is 1214.4 microliter 12. The particle-rate is 71 particles passing the laser beam/second 13. The used sample flow rate is 5.0606 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 5 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 34.7 C 18. Temperature sensor Sheath is : 33.2 C 19. Temperature sensor Sheath is : 35.2 C 20. Temperature sensor System is : 31.3 C 21. Pressure sensor difference is : -232 mBar 22. Pressure sensor absolute is : 223 mBar 23. Sample pump 1: Last calibration of the sample pump was : 12-Oct-2020 24. Alignment check 1 by curvature detector is 1.1973 (1=perfect) 25. Alignment check 2 by curvature detector is 38% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 3 (0% of 16279 measured particles) 28. FWS: Maximum signal height found is 13952 mV 29. FWS: Nr of particles with signal height <10 mV is high (13150 part. (81 %)) 30. SWS HS: Nr of saturated signals is 3 (0% of 16279 measured particles) 31. SWS HS: Maximum signal height found is 7488 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (12462 part. (77 %)) 33. FL Yellow HS: Nr of saturated signals is 0 (0% of 16279 measured particles) 34. FL Yellow HS: Maximum signal height found is 4160 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (15133 part. (93 %)) 36. FL Red HS: Nr of saturated signals is 1 (0% of 16279 measured particles) 37. FL Red HS: Maximum signal height found is 7488 mV 38. FL Red HS: Nr of particles with signal height <10 mV is high (13481 part. (83 %)) 39. SWS HS: Nr of block shaped signals is 403 (2.4756%) 40. FWS: Nr of block shaped signals is 292 (1.7937%) 41. FL Yellow HS: Nr of block shaped signals is 10 (0.061429%) 42. Suspect nr: 0=low, 10=high 43. Maximum FWS: suspect = 0 44. Maximum SWS HS: suspect = 1 45. Maximum FL Yellow HS: suspect = 0 46. Maximum FL Red HS: suspect = 1 47. SWS: PMT level is 54 48. Fl Yellow: PMT level is 90 49. FL Red: PMT level is 95 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (12-Oct-2020) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.23 Warning: FWS-L and -R might indicate an air bubble problem 38% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FWS : Nr of particles with signal height <10 mV is high (81 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: SWS HS : Nr of particles with signal height <10 mV is high (77 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (93 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (83 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)