EasyClus check & Info of last file ----- Processed at : 05-Aug-2024 06:02:45 ----- 1. The cytometric analysis is performed on machine LOG II 2. The CytoBuoy instrument serial-nr is CS-2019-94 3. The CytoUSB software used is CytoUSB Version: 5.11.1.6 4. The filename is cont-pico_flr02.00_05uls_03min 2024-08-05 05h59.cyz 5. The analysis is performed on 05-08-2024 time 05:59:23 6. The number of measured particles is 14041 7. The total concentration found (e.g. only SWS trigg.) is 16 particles/microliter 8. The concentration found (all used triggers) is 16 particles/microliter 9. The preconcentration (before analysis) found (all used triggers) is 16 particles/microliter 10. The flush time found (between samples) is 40 seconds 11. The effective analysis volume is 894.8068 microliter 12. The pumped volume is 896.4 microliter 13. The particle-rate is 78 particles passing the laser beam/second 14. The used sample flow rate is 4.9817 microliter/second 15. Trigger 1: FL Red at threshold level 2 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 31.5 C 18. Temperature sensor Sheath is : 30.6 C 19. Temperature sensor Laser is : 30.4 C 20. Temperature sensor System is : 27.7 C 21. Pressure sensor difference is : -335 mBar 22. Pressure sensor absolute is : 317 mBar 23. Sample pump 1: Last calibration of the sample pump was : 14-Apr-2022 24. Alignment check 1 by curvature detector is 0.80129 (1=perfect) 25. Alignment check 2 by curvature detector is 80.3% (>70%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 1 (0% of 14041 measured particles) 28. FWS: Maximum signal height found is 14944 mV 29. FWS: Nr of particles with signal height <10 mV is high (575 part. (4 %)) 30. SWS HS: Nr of saturated signals is 44 (0% of 14041 measured particles) 31. SWS HS: Maximum signal height found is 7616 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (171 part. (1 %)) 33. FL Yellow HS: Nr of saturated signals is 0 (0% of 14041 measured particles) 34. FL Yellow HS: Maximum signal height found is 512 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (13815 part. (98 %)) 36. FL Orange HS: Nr of saturated signals is 0 (0% of 14041 measured particles) 37. FL Orange HS: Maximum signal height found is 312 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (13948 part. (99 %)) 39. FL Red HS: Nr of saturated signals is 1 (0% of 14041 measured particles) 40. FL Red HS: Maximum signal height found is 5696 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (7657 part. (55 %)) 42. SWS HS: Nr of block shaped signals is 102 (0.72644%) 43. FWS: Nr of block shaped signals is 70 (0.49854%) 44. FL Yellow HS: Nr of block shaped signals is 1 (0.007122%) 45. Suspect nr: 0=low, 10=high 46. Maximum FWS: suspect = 0 47. Maximum SWS HS: suspect = 0 48. Maximum FL Yellow HS: suspect = 3 49. Maximum FL Orange HS: suspect = 3 50. Maximum FL Red HS: suspect = 0 51. SWS: PMT level is 60 52. FL Yellow: PMT level is 90 53. Fl Orange: PMT level is 85 54. FL Red: PMT level is 85 -------------------------- Warnings info -------------------------- 1.04 Warning: Your flush time (40 seconds) is quite small 1.04.1 Solution - Flush longer e.g. 80 sec. 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (14-Apr-2022) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (98 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.26 Warning: FL Orange HS: Biggest particles found is only 312 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Orange HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (99 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (55 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Yellow HS: suspect = 3 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data' 1.29.2: Maximum FL Orange HS: suspect = 3 1.29.12 Solution - Particle rate too high? 1.29.22 Solution - Trigger too sensitive? 1.29.32 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'