EasyClus check & Info of last file ----- Processed at : 17-Oct-2022 02:01:04 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.11.1.6 3. The filename is CGFS-continu_flr3_5ul_8min 2022-10-16 13h39.cyz 4. The analysis is performed on 16-10-2022 time 13:39:28 5. The number of measured particles is 59401 6. The total concentration found (e.g. only SWS trigg.) is 29 particles/microliter 7. The concentration found (all used triggers) is 29 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 29 particles/microliter 9. The flush time found (between samples) is 60 seconds 10. The effective analysis volume is 2066.0523 microliter 11. The pumped volume is 2068.8 microliter 12. The particle-rate is 124 particles passing the laser beam/second 13. The used sample flow rate is 4.3129 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 3 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 26.8 C 18. Temperature sensor Sheath is : 25.9 C 19. Temperature sensor Sheath is : 24.4 C 20. Temperature sensor System is : 21.2 C 21. Pressure sensor difference is : -285 mBar 22. Pressure sensor absolute is : 136 mBar 23. Sample pump 1: Last calibration of the sample pump was : 07-Mar-2022 24. Alignment check 1 by curvature detector is NaN (1=perfect) 25. Alignment check 2 by curvature detector is 0% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 1 (0% of 59401 measured particles) 28. FWS: Maximum signal height found is 14720 mV 29. FWS: Nr of particles with signal height <10 mV is high (10514 part. (18 %)) 30. SWS HS: Nr of saturated signals is 4918 (0% of 59401 measured particles) 31. SWS HS: Maximum signal height found is 7488 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (6089 part. (10 %)) 33. FL Yellow HS: Nr of saturated signals is 1 (0% of 59401 measured particles) 34. FL Yellow HS: Maximum signal height found is 6432 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (46291 part. (78 %)) 36. FL Orange HS: Nr of saturated signals is 1 (0% of 59401 measured particles) 37. FL Orange HS: Maximum signal height found is 7456 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (51007 part. (86 %)) 39. FL Red HS: Nr of saturated signals is 10 (0% of 59401 measured particles) 40. FL Red HS: Maximum signal height found is 7488 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (24141 part. (41 %)) 42. SWS HS: Nr of block shaped signals is 4038 (6.7979%) 43. FWS: Nr of block shaped signals is 2326 (3.9158%) 44. FL Yellow HS: Nr of block shaped signals is 3 (0.0050504%) 45. Suspect nr: 0=low, 10=high 46. Maximum FWS: suspect = 0 47. Maximum SWS HS: suspect = 0 48. Maximum FL Yellow HS: suspect = 1 49. Maximum FL Orange HS: suspect = 1 50. Maximum FL Red HS: suspect = 0 51. SWS: PMT level is 60 52. FL Yellow: PMT level is 90 53. Fl Orange: PMT level is 85 54. FL Red: PMT level is 85 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (07-Mar-2022) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.23 Warning: FWS-L and -R might indicate an air bubble problem 0% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (78 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (86 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.28 Warning: SWS HS: A relative large amount of particles are (rather) block shaped (7%) 1.28.1 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.28.2 Solution - Decrease, set lower SWS HS PMT voltage in CytoUSB (make less sensitive)