----- Processed at : 28-Jul-2023 12:25:06 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.11.1.6 3. The filename is APERO_Pico_continu_COURT_flr2_10ul_5min 2023-06-26 15h49.cyz 4. The analysis is performed on 26-06-2023 time 15:50:27 5. The number of measured particles is 10030 6. The total concentration found (e.g. only SWS trigg.) is 4 particles/microliter 7. The concentration found (all used triggers) is 4 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 4 particles/microliter 9. The flush time found (between samples) is 60 seconds 10. The effective analysis volume is 2854.0082 microliter 11. The pumped volume is 2856 microliter 12. The particle-rate is 33 particles passing the laser beam/second 13. The used sample flow rate is 9.5194 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 2 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 28.7 C 18. Temperature sensor Sheath is : 0.0 C 19. Temperature sensor Sheath is : 26.8 C 20. Temperature sensor System is : 24.0 C 21. Pressure sensor difference is : -400 mBar 22. Pressure sensor absolute is : 271 mBar 23. Sample pump 1: Last calibration of the sample pump was : 28-Févr-2023 24. Alignment check 1 by curvature detector is 0.8808 (1=perfect) 25. Alignment check 2 by curvature detector is 16% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 1 (0% of 10030 measured particles) 28. FWS: Maximum signal height found is 11776 mV 29. FWS: Nr of particles with signal height <10 mV is high (936 part. (9 %)) 30. SWS HS: Nr of saturated signals is 18 (0% of 10030 measured particles) 31. SWS HS: Maximum signal height found is 7488 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (628 part. (6 %)) 33. FL Yellow HS: Nr of saturated signals is 0 (0% of 10030 measured particles) 34. FL Yellow HS: Maximum signal height found is 824 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (9325 part. (93 %)) 36. FL Orange HS: Nr of saturated signals is 0 (0% of 10030 measured particles) 37. FL Orange HS: Maximum signal height found is 146 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (9804 part. (98 %)) 39. FL Red HS: Nr of saturated signals is 1 (0% of 10030 measured particles) 40. FL Red HS: Maximum signal height found is 5920 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (1434 part. (14 %)) 42. SWS HS: Nr of block shaped signals is 12 (0.11964%) 43. FWS: Nr of block shaped signals is 9 (0.089731%) 44. Suspect nr: 0=low, 10=high 45. Maximum FWS: suspect = 0 46. Maximum SWS HS: suspect = 1 47. Maximum FL Yellow HS: suspect = 3 48. Maximum FL Orange HS: suspect = 2 49. Maximum FL Red HS: suspect = 1 50. SWS: PMT level is 60 51. FL Yellow: PMT level is 90 52. Fl Orange: PMT level is 85 53. FL Red: PMT level is 85 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (28-Févr-2023) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.23 Warning: FWS-L and -R might indicate an air bubble problem 16% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (93 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.26 Warning: FL Orange HS: Biggest particles found is only 146 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Orange HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (98 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Yellow HS: suspect = 3 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'