EasyClus check & Info of last file ----- Processed at : 30-Jul-2023 07:40:46 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.11.1.6 3. The filename is APERO_Micro_continu_flr3_15ul_10min 2023-07-11 13h45.cyz 4. The analysis is performed on 11-07-2023 time 13:45:30 5. The number of measured particles is 51806 6. The total concentration found (e.g. only SWS trigg.) is 6 particles/microliter 7. The concentration found (all used triggers) is 6 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 6 particles/microliter 9. The flush time found (between samples) is 60 seconds 10. The effective analysis volume is 8581.1964 microliter 11. The pumped volume is 8586 microliter 12. The particle-rate is 86 particles passing the laser beam/second 13. The used sample flow rate is 14.3093 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 3 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 28.3 C 18. Temperature sensor Sheath is : 0.0 C 19. Temperature sensor Sheath is : 26.5 C 20. Temperature sensor System is : 23.6 C 21. Pressure sensor difference is : -402 mBar 22. Pressure sensor absolute is : 292 mBar 23. Sample pump 1: Last calibration of the sample pump was : 28-Févr-2023 24. Alignment check 1 by curvature detector is 0.58666 (1=perfect) 25. Alignment check 2 by curvature detector is 8% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 1 (0% of 51806 measured particles) 28. FWS: Maximum signal height found is 11861 mV 29. FWS: Nr of particles with signal height <10 mV is high (6949 part. (13 %)) 30. SWS HS: Nr of saturated signals is 124 (0% of 51806 measured particles) 31. SWS HS: Maximum signal height found is 7488 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (937 part. (2 %)) 33. FL Yellow HS: Nr of saturated signals is 2 (0% of 51806 measured particles) 34. FL Yellow HS: Maximum signal height found is 7488 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (42708 part. (82 %)) 36. FL Orange HS: Nr of saturated signals is 0 (0% of 51806 measured particles) 37. FL Orange HS: Maximum signal height found is 3424 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (51423 part. (99 %)) 39. FL Red HS: Nr of saturated signals is 0 (0% of 51806 measured particles) 40. FL Red HS: Maximum signal height found is 3456 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (26224 part. (51 %)) 42. FWS: Nr of block shaped signals is 73 (0.14091%) 43. SWS HS: Nr of block shaped signals is 73 (0.14091%) 44. FL Yellow HS: Nr of block shaped signals is 2 (0.0038606%) 45. Suspect nr: 0=low, 10=high 46. Maximum FWS: suspect = 0 47. Maximum SWS HS: suspect = 0 48. Maximum FL Yellow HS: suspect = 2 49. Maximum FL Orange HS: suspect = 2 50. Maximum FL Red HS: suspect = 0 51. SWS: PMT level is 60 52. FL Yellow: PMT level is 90 53. Fl Orange: PMT level is 85 54. FL Red: PMT level is 85 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (28-Févr-2023) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.22 Warning: FWS-L and -R detector signals are quite different (ratio 0.58666(1=perfect)) and might indicate an alignment problem 1.22.1 Solution - Probably you have an air bubble in your system. Pinch valve to get rid of it 1.22.2 Solution - Improve alignment of FWS-L and -R detector towards sample core 1.23 Warning: FWS-L and -R might indicate an air bubble problem 8% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (82 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (99 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (51 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)