EasyClus check & Info of last file ----- Processed at : 30-Nov-2022 18:55:41 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.9.4.8 3. The filename is AMAZOMIX_Picophyto_clear_waters_continu 2021-09-22 00h40.cyz 4. The analysis is performed on 22-09-2021 time 00:43:20 5. The number of measured particles is 84285 6. The total concentration found (e.g. only SWS trigg.) is 1368 particles/microliter 7. The concentration found (all used triggers) is 41 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 41 particles/microliter 9. The flush time found (between samples) is 70 seconds 10. The effective analysis volume is 2041.9735 microliter 11. The pumped volume is 2097.6 microliter 12. The particle-rate is 5977 particles passing the laser beam/second 13. The used sample flow rate is 4.3703 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 2 mV 16. Trigger 2: Length SWS at threshold level 1 17. Trigger 3: Maximum SWS at threshold level 3 18. Trigger 4: Maximum FWS at threshold level 2 19. Temperature sensor PMT is : 33.8 C 20. Temperature sensor Sheath is : 33.0 C 21. Temperature sensor Sheath is : 33.1 C 22. Temperature sensor System is : 29.9 C 23. Pressure sensor difference is : -244 mBar 24. Pressure sensor absolute is : 197 mBar 25. Sample pump 1: Last calibration of the sample pump was : 05-Mai-2021 26. Alignment check 1 by curvature detector is 0.82639 (1=perfect) 27. Alignment check 2 by curvature detector is 21% (100%=perfect) 28. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 29. FWS: Nr of saturated signals is 454 (0% of 84285 measured particles) 30. FWS: Maximum signal height found is 14976 mV 31. FWS: Nr of particles with signal height <10 mV is high (53701 part. (64 %)) 32. SWS HS: Nr of saturated signals is 704 (0% of 84285 measured particles) 33. SWS HS: Maximum signal height found is 7488 mV 34. SWS HS: Nr of particles with signal height <10 mV is high (2687 part. (3 %)) 35. FL Yellow HS: Nr of saturated signals is 0 (0% of 84285 measured particles) 36. FL Yellow HS: Maximum signal height found is 4160 mV 37. FL Yellow HS: Nr of particles with signal height <10 mV is high (37509 part. (45 %)) 38. FL Orange HS: Nr of saturated signals is 0 (0% of 84285 measured particles) 39. FL Orange HS: Maximum signal height found is 1296 mV 40. FL Orange HS: Nr of particles with signal height <10 mV is high (83423 part. (99 %)) 41. FL Red HS: Nr of saturated signals is 5 (0% of 84285 measured particles) 42. FL Red HS: Maximum signal height found is 7488 mV 43. FL Red HS: Nr of particles with signal height <10 mV is high (63886 part. (76 %)) 44. SWS HS: Nr of block shaped signals is 3333 (3.9544%) 45. FWS: Nr of block shaped signals is 2920 (3.4644%) 46. FL Yellow HS: Nr of block shaped signals is 242 (0.28712%) 47. Suspect nr: 0=low, 10=high 48. Maximum FWS: suspect = 2 49. Maximum SWS HS: suspect = 0 50. Maximum FL Yellow HS: suspect = 0 51. Maximum FL Orange HS: suspect = 1 52. Maximum FL Red HS: suspect = 0 53. SWS: PMT level is 65 54. FL Yellow: PMT level is 105 55. Fl Orange: PMT level is 95 56. FL Red: PMT level is 100 -------------------------- Warnings info -------------------------- 1.07 Warning: Analysis with high particle rate (5977), meaning you have higher chance of coincidence 1.07.1 Solution - Decrease, use lower sample rate 1.07.2 Solution - Is threshold in noise level? Increase threshold and find out of particle rate is suddenly lower and quite stable 1.07.3 Solution - Is the sheath fluid clean enough? 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (05-Mai-2021) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.23 Warning: FWS-L and -R might indicate an air bubble problem 21% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FWS : Nr of particles with signal height <10 mV is high (64 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (99 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (76 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)