EasyClus check & Info of last file ----- Processed at : 15-Jun-2021 11:08:20 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.9.4.8 3. The filename is AMATLANTE_flr3_5ul_8min 2021-05-22 13h41 - start higher PMT.cyz 4. The analysis is performed on 22-05-2021 time 13:43:08 5. The number of measured particles is 8725 6. The total concentration found (e.g. only SWS trigg.) is 4 particles/microliter 7. The concentration found (all used triggers) is 4 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 4 particles/microliter 9. The flush time found (between samples) is 70 seconds 10. The effective analysis volume is 2392.4687 microliter 11. The pumped volume is 2433.6 microliter 12. The particle-rate is 18 particles passing the laser beam/second 13. The used sample flow rate is 5.0661 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 3 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 30.1 C 18. Temperature sensor Sheath is : 29.4 C 19. Temperature sensor Sheath is : 27.6 C 20. Temperature sensor System is : 24.1 C 21. Pressure sensor difference is : -257 mBar 22. Pressure sensor absolute is : 197 mBar 23. Sample pump 1: Last calibration of the sample pump was : 05-May-2021 24. Alignment check 1 by curvature detector is 0.92819 (1=perfect) 25. Alignment check 2 by curvature detector is 91% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 1 (0% of 8725 measured particles) 28. FWS: Maximum signal height found is 14848 mV 29. FWS: Nr of particles with signal height <10 mV is high (625 part. (7 %)) 30. SWS HS: Nr of saturated signals is 70 (1% of 8725 measured particles) 31. SWS HS: Maximum signal height found is 7488 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (364 part. (4 %)) 33. FL Yellow HS: Nr of saturated signals is 0 (0% of 8725 measured particles) 34. FL Yellow HS: Maximum signal height found is 928 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (6067 part. (70 %)) 36. FL Orange HS: Nr of saturated signals is 0 (0% of 8725 measured particles) 37. FL Orange HS: Maximum signal height found is 20 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (8717 part. (100 %)) 39. FL Red HS: Nr of saturated signals is 3 (0% of 8725 measured particles) 40. FL Red HS: Maximum signal height found is 7488 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (4525 part. (52 %)) 42. SWS HS: Nr of block shaped signals is 401 (4.596%) 43. FWS: Nr of block shaped signals is 389 (4.4585%) 44. FL Yellow HS: Nr of block shaped signals is 29 (0.33238%) 45. Suspect nr: 0=low, 10=high 46. Maximum FWS: suspect = 2 47. Maximum SWS HS: suspect = 1 48. Maximum FL Yellow HS: suspect = 3 49. Maximum FL Orange HS: suspect = 1 50. Maximum FL Red HS: suspect = 1 51. SWS: PMT level is 60 52. FL Yellow: PMT level is 90 53. Fl Orange: PMT level is 90 54. FL Red: PMT level is 90 -------------------------- Warnings info -------------------------- 1.01 Warning: The number of saved particles in this analysis is quite low and 1.01 Warning: you might miss (statistically) bigger particles in your sample 1.01.1 Solution - Increase number of measured particles. Increase setted time OR after setted number of analyzed particles (in CytoUSB) 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (70 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.26 Warning: FL Orange HS: Biggest particles found is only 20 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Orange HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (100 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (52 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Yellow HS: suspect = 3 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'