EasyClus check & Info of last file ----- Processed at : 06-Aug-2021 22:57:43 ----- 1. The cytometric analysis is performed on machine LOG I 2. The CytoUSB software used is CytoUSB Version: 5.9.4.8 3. The filename is AMATLANTE_flr2_5ul_8min_reajusted 2021-07-15 19h19.cyz 4. The analysis is performed on 15-07-2021 time 19:20:56 5. The number of measured particles is 24687 6. The total concentration found (e.g. only SWS trigg.) is 31 particles/microliter 7. The concentration found (all used triggers) is 10 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 10 particles/microliter 9. The flush time found (between samples) is 70 seconds 10. The effective analysis volume is 2468.9092 microliter 11. The pumped volume is 2486.4 microliter 12. The particle-rate is 161 particles passing the laser beam/second 13. The used sample flow rate is 5.1821 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 2 mV 16. Trigger 2: Length SWS at threshold level 1 17. Trigger 3: Maximum SWS at threshold level 3 18. Trigger 4: Maximum FWS at threshold level 2 19. Temperature sensor PMT is : 30.9 C 20. Temperature sensor Sheath is : 29.7 C 21. Temperature sensor Sheath is : 28.5 C 22. Temperature sensor System is : 25.2 C 23. Pressure sensor difference is : -252 mBar 24. Pressure sensor absolute is : 191 mBar 25. Sample pump 1: Last calibration of the sample pump was : 05-May-2021 26. Alignment check 1 by curvature detector is 0.90146 (1=perfect) 27. Alignment check 2 by curvature detector is 66% (100%=perfect) 28. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 29. FWS: Nr of saturated signals is 263 (1% of 24687 measured particles) 30. FWS: Maximum signal height found is 14976 mV 31. FWS: Nr of particles with signal height <10 mV is high (6249 part. (25 %)) 32. SWS HS: Nr of saturated signals is 281 (1% of 24687 measured particles) 33. SWS HS: Maximum signal height found is 7488 mV 34. SWS HS: Nr of particles with signal height <10 mV is high (1102 part. (4 %)) 35. FL Yellow HS: Nr of saturated signals is 0 (0% of 24687 measured particles) 36. FL Yellow HS: Maximum signal height found is 2624 mV 37. FL Yellow HS: Nr of particles with signal height <10 mV is high (14389 part. (58 %)) 38. FL Orange HS: Nr of saturated signals is 0 (0% of 24687 measured particles) 39. FL Orange HS: Maximum signal height found is 43 mV 40. FL Orange HS: Nr of particles with signal height <10 mV is high (24674 part. (100 %)) 41. FL Red HS: Nr of saturated signals is 8 (0% of 24687 measured particles) 42. FL Red HS: Maximum signal height found is 7488 mV 43. FL Red HS: Nr of particles with signal height <10 mV is high (17805 part. (72 %)) 44. SWS HS: Nr of block shaped signals is 1056 (4.2776%) 45. FWS: Nr of block shaped signals is 929 (3.7631%) 46. FL Yellow HS: Nr of block shaped signals is 97 (0.39292%) 47. Suspect nr: 0=low, 10=high 48. Maximum FWS: suspect = 2 49. Maximum SWS HS: suspect = 0 50. Maximum FL Yellow HS: suspect = 2 51. Maximum FL Orange HS: suspect = 0 52. Maximum FL Red HS: suspect = 0 53. SWS: PMT level is 65 54. FL Yellow: PMT level is 105 55. Fl Orange: PMT level is 95 56. FL Red: PMT level is 100 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.23 Warning: FWS-L and -R might indicate an air bubble problem 66% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (58 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.26 Warning: FL Orange HS: Biggest particles found is only 43 mV. Detector sensitivity might be too low. 1.26.1 Solution - Increase, set higher FL Orange HS PMT voltage in CytoUSB (make more sensitive) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (100 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (72 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)