LL_ZI_sws15flr6_3ulIMG_600s 2016-06-14 19u03.cyz ----- Processed at : 17-Jun-2016 12:12:53 ----- 1. The cytometric analysis is performed on machine LelystadII 2. The CytoUSB software used is CytoUSB Version: 5.7.5.5 3. The filename is LL_ZI_sws15flr6_3ulIMG_600s 2016-06-14 19u03.cyz 4. The analysis is performed on 14-06-2016 time 19:03:54 5. The number of measured particles is 15336 6. The total concentration found (e.g. only SWS trigg.) is 56 particles/microliter 7. The concentration found (all used triggers) is 15 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 14 particles/microliter 9. The flush time found (between samples) is 90 seconds 10. The effective analysis volume is 1038.1247 microliter 11. The pumped volume is 1150.62 microliter 12. The particle-rate is 168 particles passing the laser beam/second 13. The used sample flow rate is 3.0184 microliter/second 14. The used block size memory is 64 kB 15. Trigger 1: SWS HS at threshold level 15 mV 16. Trigger 2: Maximum FL Red HS at threshold level 6 mV 17. Temperature sensor PMT is : 32 C 18. Temperature sensor Sheath is : 26 C 19. Temperature sensor Sheath is : 32 C 20. Pressure sensor difference is : -267 mBar 21. Pressure sensor absolute is : 225 mBar 22. Sample pump 1: Last calibration of the sample pump was : 18-Apr-2016 23. Alignment check 1 by curvature detector is 0.89543 (1=perfect) 24. Alignment check 2 by curvature detector is 80% (100%=perfect) 25. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 26. FWS: Nr of saturated signals is 1 (0% of 15336 measured particles) 27. FWS: Maximum signal height found is 9650 mV 28. FWS: Nr of particles with signal height <10 mV is high (57 part. (0 %)) 29. SWS HS: Nr of saturated signals is 10 (0% of 15336 measured particles) 30. SWS HS: Maximum signal height found is 5000 mV 31. SWS HS: Nr of particles with signal height <10 mV is high (2 part. (0 %)) 32. FL Yellow HS: Nr of saturated signals is 10 (0% of 15336 measured particles) 33. FL Yellow HS: Maximum signal height found is 5000 mV 34. FL Yellow HS: Nr of particles with signal height <10 mV is high (14969 part. (98 %)) 35. FL Orange HS: Nr of saturated signals is 3 (0% of 15336 measured particles) 36. FL Orange HS: Maximum signal height found is 5000 mV 37. FL Orange HS: Nr of particles with signal height <10 mV is high (14907 part. (97 %)) 38. FL Red HS: Nr of saturated signals is 1 (0% of 15336 measured particles) 39. FL Red HS: Maximum signal height found is 5000 mV 40. FL Red HS: Nr of particles with signal height <10 mV is high (4229 part. (28 %)) 41. FL Yellow HS: Nr of block shaped signals is 22 (0.14345%) 42. SWS HS: Nr of block shaped signals is 4 (0.026082%) 43. FWS: Nr of block shaped signals is 2 (0.013041%) 44. SWS HS: PMT level is 50 45. SWS LS: PMT level is 32 46. FL Yellow LS: PMT level is 61 47. FL Red LS: PMT level is 63 48. FL Yellow HS: PMT level is 83 49. FL Orange HS: PMT level is 80 50. FL Red HS: PMT level is 93 51. IIF: Imaging in Flow pictures available. Nr of images is 159 -------------------------- Warnings info -------------------------- 1.20 Warning: FL Yellow LS: HS-LS ratio is 65 and too low. 1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.20 Warning: FL Red LS: HS-LS ratio is 234 and too high. 1.20.1 Solution - Put LS PMT higher. Ratio HS/LS should be around 100. 1.23 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (98 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.23 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (97 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)