EasyClus check & Info of last file ----- Processed at : 29-Nov-2021 12:30:14 ----- 1. The cytometric analysis is performed on machine Markermeer 2. The CytoUSB software used is CytoUSB Version: 5.9.4.4 3. The filename is CS2019-91 MW beads 2021-11-29 12h18.cyz 4. The analysis is performed on 29-11-2021 time 12:18:54 5. The number of measured particles is 11740 6. The total concentration found (e.g. only SWS trigg.) is 741 particles/microliter 7. The concentration found (all used triggers) is 38 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 38 particles/microliter 9. The flush time found (between samples) is 130 seconds 10. The effective analysis volume is 305.6026 microliter 11. The pumped volume is 308.4 microliter 12. The particle-rate is 1908 particles passing the laser beam/second 13. The used sample flow rate is 2.573 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: SWS HS at threshold level 15 mV 16. Trigger 2: Maximum FL Red HS at threshold level 6 17. Temperature sensor PMT is : 10.0 C 18. Temperature sensor Sheath is : 8.9 C 19. Temperature sensor Sheath is : 9.2 C 20. Temperature sensor System is : 7.1 C 21. Pressure sensor difference is : -299 mBar 22. Pressure sensor absolute is : 349 mBar 23. External battery Voltage is : 13.0 Volt 24. Sample pump 1: Last calibration of the sample pump was : 24-Feb-2021 25. Alignment check 1 by curvature detector is 1.0522 (1=perfect) 26. Alignment check 2 by curvature detector is 98% (100%=perfect) 27. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 28. FWS: Nr of saturated signals is 1 (0% of 11740 measured particles) 29. FWS: Maximum signal height found is 12416 mV 30. FWS: Nr of particles with signal height <10 mV is high (250 part. (2 %)) 31. SWS HS: Nr of saturated signals is 2 (0% of 11740 measured particles) 32. SWS HS: Maximum signal height found is 7360 mV 33. SWS HS: Nr of particles with signal height <10 mV is high (0 part. (0 %)) 34. FL Yellow HS: Nr of saturated signals is 1 (0% of 11740 measured particles) 35. FL Yellow HS: Maximum signal height found is 7360 mV 36. FL Yellow HS: Nr of particles with signal height <10 mV is high (8453 part. (72 %)) 37. FL Orange HS: Nr of saturated signals is 0 (0% of 11740 measured particles) 38. FL Orange HS: Maximum signal height found is 2592 mV 39. FL Orange HS: Nr of particles with signal height <10 mV is high (7656 part. (65 %)) 40. FL Red HS: Nr of saturated signals is 3 (0% of 11740 measured particles) 41. FL Red HS: Maximum signal height found is 7360 mV 42. FL Red HS: Nr of particles with signal height <10 mV is high (4455 part. (38 %)) 43. FL Red HS: Nr of block shaped signals is 26 (0.22147%) 44. SWS HS: Nr of block shaped signals is 22 (0.18739%) 45. FWS: Nr of block shaped signals is 16 (0.13629%) 46. FL Orange HS: Nr of block shaped signals is 4 (0.034072%) 47. FL Yellow HS: Nr of block shaped signals is 2 (0.017036%) 48. Suspect nr: 0=low, 10=high 49. Maximum FWS: suspect = 0 50. Maximum SWS HS: suspect = 0 51. Maximum SWS LS: suspect = 1 52. Maximum FL Yellow HS: suspect = 3 53. Maximum FL Yellow LS: suspect = 0 54. Maximum FL Orange HS: suspect = 1 55. Maximum FL Red HS: suspect = 1 56. Maximum FL Red LS: suspect = 1 57. SWS HS: PMT level is 54 58. SWS LS: PMT level is 48 59. FL Yellow HS: PMT level is 87 60. FL Yellow LS: PMT level is 68 61. Fl Orange: PMT level is 93 62. FL Red HS: PMT level is 116 63. FL Red LS: PMT level is 81 -------------------------- Warnings info -------------------------- 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (24-Feb-2021) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.24 Warning: SWS LS: Maximum is 221 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: SWS LS: HS-LS ratio is 62 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Yellow LS: HS-LS ratio is 49 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Red LS: Maximum is 132 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Red LS: HS-LS ratio is 56 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (72 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (65 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Yellow HS: suspect = 3 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'