EasyClus check & Info of last file ----- Processed at : 20-Apr-2022 15:21:38 ----- 1. The cytometric analysis is performed on machine RWS Systeem 2 2. The CytoUSB software used is CytoUSB Version: 5.8.3.31 3. The filename is CS_2019_92_prot_1 2021-01-28 14h21.cyz 4. The analysis is performed on 28-01-2021 time 14:23:37 5. The number of measured particles is 8996 6. The total concentration found (e.g. only SWS trigg.) is 149 particles/microliter 7. The concentration found (all used triggers) is 149 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 149 particles/microliter 9. The flush time found (between samples) is 130 seconds 10. The effective analysis volume is 60.5193 microliter 11. The pumped volume is 60.6 microliter 12. The particle-rate is 150 particles passing the laser beam/second 13. The used sample flow rate is 1.0101 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: SWS HS at threshold level 15 mV 16. Trigger 2: not used at threshold level -999 17. Temperature sensor PMT is : 32.1 C 18. Temperature sensor Sheath is : 31.6 C 19. Temperature sensor Sheath is : 30.6 C 20. Temperature sensor System is : 26.4 C 21. Pressure sensor difference is : -226 mBar 22. Pressure sensor absolute is : 171 mBar 23. Sample pump 1: Last calibration of the sample pump was : 26-Okt-2020 24. Alignment check 1 by curvature detector is 1.1865 (1=perfect) 25. Alignment check 2 by curvature detector is 35% (100%=perfect) 26. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 27. FWS: Nr of saturated signals is 1 (0% of 8996 measured particles) 28. FWS: Maximum signal height found is 6592 mV 29. FWS: Nr of particles with signal height <10 mV is high (4591 part. (51 %)) 30. SWS HS: Nr of saturated signals is 1 (0% of 8996 measured particles) 31. SWS HS: Maximum signal height found is 7104 mV 32. SWS HS: Nr of particles with signal height <10 mV is high (0 part. (0 %)) 33. FL Yellow HS: Nr of saturated signals is 0 (0% of 8996 measured particles) 34. FL Yellow HS: Maximum signal height found is 2208 mV 35. FL Yellow HS: Nr of particles with signal height <10 mV is high (8977 part. (100 %)) 36. FL Orange HS: Nr of saturated signals is 0 (0% of 8996 measured particles) 37. FL Orange HS: Maximum signal height found is 2032 mV 38. FL Orange HS: Nr of particles with signal height <10 mV is high (8945 part. (99 %)) 39. FL Red HS: Nr of saturated signals is 0 (0% of 8996 measured particles) 40. FL Red HS: Maximum signal height found is 1936 mV 41. FL Red HS: Nr of particles with signal height <10 mV is high (8653 part. (96 %)) 42. SWS HS: Nr of block shaped signals is 16 (0.17786%) 43. FWS: Nr of block shaped signals is 3 (0.033348%) 44. Suspect nr: 0=low, 10=high 45. Maximum FWS: suspect = 1 46. Maximum SWS HS: suspect = 1 47. Maximum SWS LS: suspect = 2 48. Maximum FL Yellow HS: suspect = 0 49. Maximum FL Yellow LS: suspect = 4 50. Maximum FL Orange HS: suspect = 0 51. Maximum FL Red HS: suspect = 0 52. Maximum FL Red LS: suspect = 0 53. SWS HS: PMT level is 56 54. SWS LS: PMT level is 46 55. FL Yellow HS: PMT level is 85 56. FL Yellow LS: PMT level is 68 57. Fl Orange: PMT level is 77 58. FL Red HS: PMT level is 110 59. FL Red LS: PMT level is 91 60. IIF: Imaging in Flow pictures available. Nr of images is 304 -------------------------- Warnings info -------------------------- 1.01 Warning: The number of saved particles in this analysis is quite low and 1.01 Warning: you might miss (statistically) bigger particles in your sample 1.01.1 Solution - Increase number of measured particles. Increase setted time OR after setted number of analyzed particles (in CytoUSB) 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume. (Only older CytoSenses) 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) OR set to 'auto' 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (26-Okt-2020) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.23 Warning: FWS-L and -R might indicate an air bubble problem 35% (100%=perfect) 1.23.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.24 Warning: SWS LS: Maximum is 135 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: SWS LS: HS-LS ratio is 46 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Red LS: HS-LS ratio is 23 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.27 Warning: FWS : Nr of particles with signal height <10 mV is high (51 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (100 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (99 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (96 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Yellow LS: suspect = 4 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'