LL_Cend12_16_sws15flr6_3u_lIMG_600s 2016-06-26 12u21.cyz ----- Processed at : 30-Jun-2016 10:38:50 ----- 1. The cytometric analysis is performed on machine LelystadII 2. The CytoUSB software used is CytoUSB Version: 5.7.5.5 3. The filename is LL_Cend12_16_sws15flr6_3u_lIMG_600s 2016-06-26 12u21.cyz 4. The analysis is performed on 26-06-2016 time 12:21:51 5. The number of measured particles is 13346 6. The total concentration found (e.g. only SWS trigg.) is 104 particles/microliter 7. The concentration found (all used triggers) is 9 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 11 particles/microliter 9. The flush time found (between samples) is 120 seconds 10. The effective analysis volume is 1556.9588 microliter 11. The pumped volume is 1812 microliter 12. The particle-rate is 313 particles passing the laser beam/second 13. The used sample flow rate is 3.0184 microliter/second 14. The used block size memory is 64 kB 15. Trigger 1: SWS HS at threshold level 15 mV 16. Trigger 2: Maximum FL Red HS at threshold level 6 mV 17. Temperature sensor PMT is : 32 C 18. Temperature sensor Sheath is : 26 C 19. Temperature sensor Sheath is : 32 C 20. Pressure sensor difference is : -272 mBar 21. Pressure sensor absolute is : 180 mBar 22. Sample pump 1: Last calibration of the sample pump was : 18-Apr-2016 23. Alignment check 1 by curvature detector is 0.89551 (1=perfect) 24. Alignment check 2 by curvature detector is 80% (100%=perfect) 25. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 26. FWS: Nr of saturated signals is 1 (0% of 13346 measured particles) 27. FWS: Maximum signal height found is 5528 mV 28. FWS: Nr of particles with signal height <10 mV is high (127 part. (1 %)) 29. SWS HS: Nr of saturated signals is 18 (0% of 13346 measured particles) 30. SWS HS: Maximum signal height found is 5000 mV 31. SWS HS: Nr of particles with signal height <10 mV is high (0 part. (0 %)) 32. FL Yellow HS: Nr of saturated signals is 15 (0% of 13346 measured particles) 33. FL Yellow HS: Maximum signal height found is 5000 mV 34. FL Yellow HS: Nr of particles with signal height <10 mV is high (12551 part. (94 %)) 35. FL Orange HS: Nr of saturated signals is 15 (0% of 13346 measured particles) 36. FL Orange HS: Maximum signal height found is 5000 mV 37. FL Orange HS: Nr of particles with signal height <10 mV is high (12397 part. (93 %)) 38. FL Red HS: Nr of saturated signals is 9 (0% of 13346 measured particles) 39. FL Red HS: Maximum signal height found is 5000 mV 40. FL Red HS: Nr of particles with signal height <10 mV is high (1418 part. (11 %)) 41. FL Yellow HS: Nr of block shaped signals is 31 (0.23228%) 42. SWS HS: Nr of block shaped signals is 18 (0.13487%) 43. FWS: Nr of block shaped signals is 1 (0.0074929%) 44. FL Orange HS: Nr of block shaped signals is 1 (0.0074929%) 45. SWS HS: PMT level is 50 46. SWS LS: PMT level is 32 47. FL Yellow LS: PMT level is 61 48. FL Red LS: PMT level is 63 49. FL Yellow HS: PMT level is 83 50. FL Orange HS: PMT level is 80 51. FL Red HS: PMT level is 93 52. IIF: Imaging in Flow pictures available. Nr of images is 157 -------------------------- Warnings info -------------------------- 1.20 Warning: FL Yellow LS: HS-LS ratio is 79 and too low. 1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.20 Warning: FL Red LS: Maximum is only 20 mV (if HS is around 4500 mV) and too low. 1.20.1 Solution - Put LS PMT higher. Maximum LS around 50 mV (if HS is around 4500 mV). 1.20 Warning: FL Red LS: HS-LS ratio is 191 and too high. 1.20.1 Solution - Put LS PMT higher. Ratio HS/LS should be around 100. 1.23 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (94 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.23 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (93 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)