EasyClus check & Info of last file ----- Processed at : 22-Dec-2024 03:17:38 ----- 1. The cytometric analysis is performed on machine Lobith 2. The CytoBuoy instrument serial-nr is CS-2021-106 3. The CytoUSB software used is CytoUSB Version: 5.11.3.1 4. LASER : OBIS LS 488-60 : Used at 50 mW 5. LASER : OBIS LS 552-60 : Used at 50 mW 6. The filename is EIJ2_SWS15_FLRed10_auto_uls_phyto_600s 2024-12-22 03h02.cyz 7. The analysis is performed on 22-12-2024 time 03:04:36 8. The number of measured particles is 11357 9. The total concentration found (e.g. only SWS trigg.) is 2900 particles/microliter 10. The concentration found (all used triggers) is 14 particles/microliter 11. The preconcentration (before analysis) found (all used triggers) is 14 particles/microliter 12. The flush time found (between samples) is 130 seconds 13. The effective analysis volume is 789.121 microliter 14. The pumped volume is 798 microliter 15. The particle-rate is 3870 particles passing the laser beam/second 16. The used sample flow rate is 1.3346 microliter/second 17. Trigger 1: SWS HS at threshold level 15 mV 18. Trigger 2: Maximum FL Red HS at threshold level 10 19. Temperature sensor PMT is : 26.4 C 20. Temperature sensor Sheath is : 21.5 C 21. Temperature sensor Laser is : 26.0 C 22. Temperature sensor System is : 24.0 C 23. Pressure sensor difference is : -426 mBar 24. Pressure sensor absolute is : 318 mBar 25. Sample pump 1: Last calibration of the sample pump was : 05-Dec-2024 26. Alignment check 1 by curvature detector is 1.0783 (1=perfect) 27. Alignment check 2 by curvature detector is 88.4% (>70%=perfect) 28. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 29. FWS: Nr of saturated signals is 1 (0% of 11357 measured particles) 30. FWS: Maximum signal height found is 14464 mV 31. FWS: Nr of particles with signal height <10 mV is high (235 part. (2 %)) 32. SWS HS: Nr of saturated signals is 1 (0% of 11357 measured particles) 33. SWS HS: Maximum signal height found is 7264 mV 34. SWS HS: Nr of particles with signal height <10 mV is high (0 part. (0 %)) 35. FL Yellow HS: Nr of saturated signals is 2 (0% of 11357 measured particles) 36. FL Yellow HS: Maximum signal height found is 7360 mV 37. FL Yellow HS: Nr of particles with signal height <10 mV is high (5272 part. (46 %)) 38. FL Orange HS: Nr of saturated signals is 2 (0% of 11357 measured particles) 39. FL Orange HS: Maximum signal height found is 7360 mV 40. FL Orange HS: Nr of particles with signal height <10 mV is high (1371 part. (12 %)) 41. FL Red HS: Nr of saturated signals is 4 (0% of 11357 measured particles) 42. FL Red HS: Maximum signal height found is 7360 mV 43. FL Red HS: Nr of particles with signal height <10 mV is high (348 part. (3 %)) 44. FL Orange HS: Nr of block shaped signals is 141 (1.2415%) 45. FL Yellow HS: Nr of block shaped signals is 16 (0.14088%) 46. SWS HS: Nr of block shaped signals is 8 (0.070441%) 47. FL Red HS: Nr of block shaped signals is 5 (0.044026%) 48. FWS: Nr of block shaped signals is 4 (0.035221%) 49. Suspect nr: 0=low, 10=high 50. Maximum FWS: suspect = 0 51. Maximum SWS HS: suspect = 1 52. Maximum SWS LS: suspect = 2 53. Maximum FL Yellow HS: suspect = 5 54. Maximum FL Yellow LS: suspect = 3 55. Maximum FL Orange HS: suspect = 5 56. Maximum FL Red HS: suspect = 2 57. Maximum FL Red LS: suspect = 2 58. SWS HS: PMT level is 49 59. SWS LS: PMT level is 48 60. FL Yellow HS: PMT level is 94 61. FL Yellow LS: PMT level is 76 62. Fl Orange: PMT level is 105 63. FL Red HS: PMT level is 110 64. FL Red LS: PMT level is 81 -------------------------- Warnings info -------------------------- 1.07 Warning: Analysis with high particle rate (3870), meaning you have higher chance of coincidence 1.07.1 Solution - Decrease, use lower sample rate 1.07.2 Solution - Is threshold in noise level? Increase threshold and find out of particle rate is suddenly lower and quite stable 1.07.3 Solution - Is the sheath fluid clean enough? 1.24 Warning: SWS LS: Maximum is 280 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Yellow LS: HS-LS ratio is 27 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Yellow LS: Maximum is only 6 mV (if HS is around 4500 mV) and too low. 1.24.1 Solution - Put LS PMT higher. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Yellow LS: HS-LS ratio is 26 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Red LS: Maximum is 103 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Red LS: HS-LS ratio is 64 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum FL Yellow HS: suspect = 5 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data' 1.29.2: Maximum FL Yellow LS: suspect = 3 1.29.12 Solution - Particle rate too high? 1.29.22 Solution - Trigger too sensitive? 1.29.32 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data' 1.29.3: Maximum FL Orange HS: suspect = 5 1.29.13 Solution - Particle rate too high? 1.29.23 Solution - Trigger too sensitive? 1.29.33 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'