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EIJ2_SWS15_FLRed6_auto_uls_IMG_600s 2021-11-30 12h04.cyz

----- Processed at : 30-Nov-2021 12:15:29 -----

1. The cytometric analysis is performed on machine LelystadII
2. The CytoUSB software used is CytoUSB Version: 5.9.1.5
3. The filename is EIJ2_SWS15_FLRed6_auto_uls_IMG_600s 2021-11-30 12h04.cyz
4. The analysis is performed on 30-11-2021 time 12:05:01
5. The number of measured particles is 98568
6. The total concentration found (e.g. only SWS trigg.) is 486 particles/microliter
7. The concentration found (all used triggers) is 181 particles/microliter
8. The preconcentration (before analysis) found (all used triggers) is 181 particles/microliter
9. The flush time found (between samples) is 110 seconds
10. The effective analysis volume is 545.1261 microliter
11. The pumped volume is 1218 microliter
12. The particle-rate is 988 particles passing the laser beam/second
13. The used sample flow rate is 2.0349 microliter/second
14. The used block size memory is 4 kB
15. Trigger 1: SWS HS at threshold level 15 mV
16. Trigger 2: Maximum FL Red HS at threshold level 6
17. Temperature sensor PMT is : 26 C
18. Temperature sensor Sheath is : 22 C
19. Temperature sensor Sheath is : 27 C
20. Pressure sensor difference is : -788 mBar
21. Pressure sensor absolute is : 667 mBar
22. Sample pump 1: Last calibration of the sample pump was : 08-Sep-2021
23. Alignment check 1 by curvature detector is 1.1061 (1=perfect)
24. Alignment check 2 by curvature detector is 47% (100%=perfect)
25. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached
26. FWS: Nr of saturated signals is 1 (0% of 98568 measured particles)
27. FWS: Maximum signal height found is 6518 mV
28. FWS: Nr of particles with signal height <10 mV is high (63808 part. (65 %))
29. SWS HS: Nr of saturated signals is 0 (0% of 98568 measured particles)
30. SWS HS: Maximum signal height found is 2659 mV
31. SWS HS: Nr of particles with signal height <10 mV is high (76 part. (0 %))
32. FL Yellow HS: Nr of saturated signals is 0 (0% of 98568 measured particles)
33. FL Yellow HS: Maximum signal height found is 3216 mV
34. FL Yellow HS: Nr of particles with signal height <10 mV is high (7684 part. (8 %))
35. FL Orange HS: Nr of saturated signals is 0 (0% of 98568 measured particles)
36. FL Orange HS: Maximum signal height found is 3487 mV
37. FL Orange HS: Nr of particles with signal height <10 mV is high (37752 part. (38 %))
38. FL Red HS: Nr of saturated signals is 3 (0% of 98568 measured particles)
39. FL Red HS: Maximum signal height found is 5000 mV
40. FL Red HS: Nr of particles with signal height <10 mV is high (96705 part. (98 %))
41. FL Yellow HS: Nr of block shaped signals is 10047 (10.193%)
42. FL Orange HS: Nr of block shaped signals is 606 (0.6148%)
43. SWS HS: Nr of block shaped signals is 134 (0.13595%)
44. FWS: Nr of block shaped signals is 8 (0.0081162%)
45. FL Red HS: Nr of block shaped signals is 7 (0.0071017%)
46. SWS LS: Nr of block shaped signals is 1 (0.0010145%)
47. Suspect nr: 0=low, 10=high
48. Maximum FWS: suspect = 0
49. Maximum SWS HS: suspect = 0
50. Maximum FL Yellow HS: suspect = 0
51. Maximum FL Orange HS: suspect = 3
52. Maximum FL Red HS: suspect = 2
53. Maximum SWS LS: suspect = 0
54. Maximum FL Yellow LS: suspect = 0
55. Maximum FL Red LS: suspect = 0
56. SWS HS: PMT level is 47
57. FL Yellow HS: PMT level is 88
58. FL Orange HS: PMT level is 86
59. FL Red HS: PMT level is 101
60. SWS LS: PMT level is 34
61. FL Yellow LS: PMT level is 65
62. FL Red LS: PMT level is 75
63. IIF: Imaging in Flow pictures available. Nr of images is 138

-------------------------- Warnings info --------------------------
1.05 Warning: Your effective analysis volume is low compared to the pumped analysis volume
1.05.1 Solution - Set blocksize memory in CytoUSB higher, used=4k (default=4k) e.g. to 8k
1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume
1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs)
1.15 Warning: Pressure difference is high.
1.15.1 Solution - Check if there is something blocking from sample inlet to sheath (filter block, tubing)
1.19 Warning: FWS-L and -R might indicate an air bubble problem 47% (100%=perfect)
1.19.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different
1.20 Warning: SWS LS: HS-LS ratio is 34 and too low.
1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100.
1.20 Warning: FL Yellow LS: HS-LS ratio is 33 and too low.
1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100.
1.20 Warning: FL Yellow LS: HS-LS ratio is 32 and too low.
1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100.
1.20 Warning: FL Red LS: HS-LS ratio is 64 and too low.
1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100.
1.23 Warning: FWS : Nr of particles with signal height <10 mV is high (65 %)
1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise)
1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample
1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)
1.23 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (98 %)
1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise)
1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample
1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)
1.24 Warning: FL Yellow HS: A relative large amount of particles are (rather) block shaped (10%)
1.24.1 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample
1.24.2 Solution - Decrease, set lower FL Yellow HS PMT voltage in CytoUSB (make less sensitive)
1.25 Warning: Suspected particles data blocks found!
1.25.1: Maximum FL Orange HS: suspect = 3
1.25.1 Solution - Particle rate too high?
1.25.1 Solution - Trigger too sensitive?
1.25.1 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'
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