EIJ_SWS15_FLRed6_auto_uls_phyto_600s 2019-11-21 08h02.cyz ----- Processed at : 21-Nov-2019 08:13:02 ----- 1. The cytometric analysis is performed on machine Eijsden 2. The CytoUSB software used is CytoUSB Version: 5.8.2.17 3. The filename is EIJ_SWS15_FLRed6_auto_uls_phyto_600s 2019-11-21 08h02.cyz 4. The analysis is performed on 21-11-2019 time 08:02:49 5. The number of measured particles is 145 6. The total concentration found (e.g. only SWS trigg.) is 0 particles/microliter 7. The concentration found (all used triggers) is 0 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 0 particles/microliter 9. The flush time found (between samples) is 130 seconds 10. The effective analysis volume is 3851.4789 microliter 11. The pumped volume is 2364 microliter 12. The particle-rate is 0 particles passing the laser beam/second 13. The used sample flow rate is 3.9382 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: SWS HS at threshold level 15 mV 16. Trigger 2: Maximum FL Red HS at threshold level 6 mV 17. Temperature sensor PMT is : 28 C 18. Temperature sensor Sheath is : 23 C 19. Temperature sensor Sheath is : 28 C 20. Pressure sensor difference is : -543 mBar 21. Pressure sensor absolute is : 350 mBar 22. Sample pump 1: Last calibration of the sample pump was : 13-Sep-2019 23. Alignment check 1 by curvature detector is 1.1534 (1=perfect) 24. Alignment check 2 by curvature detector is 16% (100%=perfect) 25. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 26. FWS: Nr of saturated signals is 0 (0% of 145 measured particles) 27. FWS: Maximum signal height found is 2 mV 28. FWS: Nr of particles with signal height <10 mV is high (145 part. (100 %)) 29. SWS HS: Nr of saturated signals is 0 (0% of 145 measured particles) 30. SWS HS: Maximum signal height found is 627 mV 31. SWS HS: Nr of particles with signal height <10 mV is high (1 part. (1 %)) 32. FL Yellow HS: Nr of saturated signals is 0 (0% of 145 measured particles) 33. FL Yellow HS: Maximum signal height found is 268 mV 34. FL Yellow HS: Nr of particles with signal height <10 mV is high (29 part. (20 %)) 35. FL Orange HS: Nr of saturated signals is 0 (0% of 145 measured particles) 36. FL Orange HS: Maximum signal height found is 196 mV 37. FL Orange HS: Nr of particles with signal height <10 mV is high (31 part. (21 %)) 38. FL Red HS: Nr of saturated signals is 0 (0% of 145 measured particles) 39. FL Red HS: Maximum signal height found is 60 mV 40. FL Red HS: Nr of particles with signal height <10 mV is high (6 part. (4 %)) 41. Suspect nr: 0=low, 10=high 42. Maximum FWS: suspect = 0 43. Maximum SWS HS: suspect = 0 44. Maximum FL Yellow HS: suspect = 0 45. Maximum FL Orange HS: suspect = 0 46. Maximum FL Red HS: suspect = 5 47. Maximum SWS LS: suspect = 0 48. Maximum FL Yellow LS: suspect = 4 49. Maximum FL Red LS: suspect = 0 50. SWS HS: PMT level is 55 51. FL Yellow HS: PMT level is 81 52. FL Orange HS: PMT level is 80 53. FL Red HS: PMT level is 100 54. SWS LS: PMT level is 39 55. FL Yellow LS: PMT level is 66 56. FL Red LS: PMT level is 74 -------------------------- Warnings info -------------------------- 1.01 Warning: The number of saved particles in this analysis is quite low and 1.01 Warning: you might miss (statistically) bigger particles in your sample 1.01.1 Solution - Increase number of measured particles. Increase setted time OR after setted number of analyzed particles (in CytoUSB) 1.03 Warning: Your preconcentration differs more than 25% from the during analysis concentration 1.03.1 Solution - Carry over between samples? > Flush longer 1.03.2 Solution - Better homogenize samples during subsampling by a gentle stirrer 1.03.3 Solution - Colonies-filaments of cells break during analysis > stir slower 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) 1.19 Warning: FWS-L and -R might indicate an air bubble problem 16% (100%=perfect) 1.19.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.20 Warning: SWS LS: HS-LS ratio is 38 and too low. 1.20.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.20 Warning: FL Yellow LS: HS-LS ratio is 999 and too high. 1.20.1 Solution - Put LS PMT higher. Ratio HS/LS should be around 100. 1.20 Warning: FL Yellow LS: HS-LS ratio is 999 and too high. 1.20.1 Solution - Put LS PMT higher. Ratio HS/LS should be around 100. 1.20 Warning: FL Red LS: HS-LS ratio is 999 and too high. 1.20.1 Solution - Put LS PMT higher. Ratio HS/LS should be around 100. 1.22 Warning: FWS: Biggest particles found is only 2 mV. Detector sensitivity might be too low. 1.22.1 Solution - Increase, set higher FWS PMT voltage in CytoUSB (make more sensitive) 1.23 Warning: FWS : Nr of particles with signal height <10 mV is high (100 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.22 Warning: FL Yellow HS: Biggest particles found is only 268 mV. Detector sensitivity might be too low. 1.22.1 Solution - Increase, set higher FL Yellow HS PMT voltage in CytoUSB (make more sensitive) 1.22 Warning: FL Orange HS: Biggest particles found is only 196 mV. Detector sensitivity might be too low. 1.22.1 Solution - Increase, set higher FL Orange HS PMT voltage in CytoUSB (make more sensitive) 1.22 Warning: FL Red HS: Biggest particles found is only 60 mV. Detector sensitivity might be too low. 1.22.1 Solution - Increase, set higher FL Red HS PMT voltage in CytoUSB (make more sensitive) 1.25 Warning: Suspected particles data blocks found! 1.25.1: Maximum FL Red HS: suspect = 5 1.25.1 Solution - Particle rate too high? 1.25.1 Solution - Trigger too sensitive? 1.25.1 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data' 1.25.2: Maximum FL Yellow LS: suspect = 4 1.25.2 Solution - Particle rate too high? 1.25.2 Solution - Trigger too sensitive? 1.25.2 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'