EasyClus check & Info of last file ----- Processed at : 01-Nov-2024 17:11:43 ----- 1. The cytometric analysis is performed on machine Cefas III 2. The CytoBuoy instrument serial-nr is CS-2022-114 3. The CytoUSB software used is CytoUSB Version: Build: 'ReleaseWebsite' , Commit: CytoUsb-v6.3.3.0-0-g9b2786abc, User=rob@Tommie, Machine=TOMMIE, ProjectPath=C:\Users\rob\Documents\Git\CytoSoftware\CytoUSB\+9b2786abc537a909b242db2a87f0202103b704b1 4. LASER : OBIS LS 488-60mW : Used at 50 mW 5. The filename is nano_cend17_24 2024-11-01 15h59.cyz 6. The analysis is performed on 01-11-2024 time 16:03:09 7. The number of measured particles is 133588 8. The total concentration found (e.g. only SWS trigg.) is 25 particles/microliter 9. The concentration found (all used triggers) is 25 particles/microliter 10. The preconcentration (before analysis) found (all used triggers) is 25 particles/microliter 11. The flush time found (between samples) is 190 seconds 12. The effective analysis volume is 5374.8885 microliter 13. The pumped volume is 5394 microliter 14. The particle-rate is 223 particles passing the laser beam/second 15. The used sample flow rate is 8.9854 microliter/second 16. Trigger 1: Fl Red at threshold level 3 mV 17. Trigger 2: not used at threshold level -999 18. Temperature sensor PMT is : 27.5 C 19. Temperature sensor Sheath is : 25.5 C 20. Temperature sensor Laser is : 26.6 C 21. Temperature sensor System is : 24.3 C 22. Pressure sensor difference is : -236 mBar 23. Pressure sensor absolute is : 281 mBar 24. Sample pump 1: Last calibration of the sample pump was : 05-Sept-2024 25. Alignment check 1 by curvature detector is 1.1158 (1=perfect) 26. Alignment check 2 by curvature detector is 73.2% (>70%=perfect) 27. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 28. FWS: Nr of saturated signals is 1 (0% of 133588 measured particles) 29. FWS: Maximum signal height found is 11328 mV 30. FWS: Nr of particles with signal height <10 mV is high (16352 part. (12 %)) 31. SWS HS: Nr of saturated signals is 1791 (0% of 133588 measured particles) 32. SWS HS: Maximum signal height found is 7488 mV 33. SWS HS: Nr of particles with signal height <10 mV is high (9395 part. (7 %)) 34. FL Yellow HS: Nr of saturated signals is 0 (0% of 133588 measured particles) 35. FL Yellow HS: Maximum signal height found is 1280 mV 36. FL Yellow HS: Nr of particles with signal height <10 mV is high (101353 part. (76 %)) 37. FL Orange HS: Nr of saturated signals is 0 (0% of 133588 measured particles) 38. FL Orange HS: Maximum signal height found is 1168 mV 39. FL Orange HS: Nr of particles with signal height <10 mV is high (129203 part. (97 %)) 40. FL Red HS: Nr of saturated signals is 1 (0% of 133588 measured particles) 41. FL Red HS: Maximum signal height found is 7488 mV 42. FL Red HS: Nr of particles with signal height <10 mV is high (69308 part. (52 %)) 43. FWS: Nr of block shaped signals is 3053 (2.2854%) 44. SWS HS: Nr of block shaped signals is 2619 (1.9605%) 45. Suspect nr: 0=low, 10=high 46. Maximum FWS: suspect = 0 47. Maximum SWS HS: suspect = 0 48. Maximum FL Yellow HS: suspect = 0 49. Maximum FL Orange HS: suspect = 0 50. Maximum FL Red HS: suspect = 0 51. Sidewards Scatter: PMT level is 60 52. Fl Yellow: PMT level is 95 53. Fl Orange: PMT level is 85 54. Fl Red: PMT level is 85 55. IIF: Imaging in Flow pictures available. Nr of images is 250 -------------------------- Warnings info -------------------------- 1.10 Warning: This threshold level is quite low and near noise 1.10 Warning: Take care you don't measure noise or in noise (this will give high particle rates but is in fact noise) 1.10.1 Solution - Increase threshold trigger level 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (76 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (97 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.27 Warning: FL Red HS : Nr of particles with signal height <10 mV is high (52 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)