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Online automatic phytoplankton monitoring at several locations performed by flowcytometry (CytoSense-CytoSub)
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nano_cend15_19 2020-02-07 22u03.cyz

----- Processed at : 26-Sep-2020 20:11:20 -----

1. The cytometric analysis is performed on machine Cefas
2. The CytoUSB software used is CytoUSB Version: 5.7.1.0
3. The filename is nano_cend15_19 2020-02-07 22u03.cyz
4. The analysis is performed on 07-02-2020 time 22:03:29
5. The number of measured particles is 67450
6. The total concentration found (e.g. only SWS trigg.) is 22 particles/microliter
7. The concentration found (all used triggers) is 22 particles/microliter
8. The preconcentration (before analysis) found (all used triggers) is 20 particles/microliter
9. The flush time found (between samples) is 180 seconds
10. The effective analysis volume is 3081.1958 microliter
11. The pumped volume is 5178 microliter
12. The particle-rate is 189 particles passing the laser beam/second
13. The used sample flow rate is 8.6324 microliter/second
14. The used block size memory is 4 kB
15. Trigger 1: FL Red at threshold level 21 mV
16. Trigger 2: not used at threshold level -999 mV
17. Temperature sensor PMT is : -999 C
18. Temperature sensor Sheath is : -999 C
19. Temperature sensor Sheath is : 22 C
20. Pressure sensor difference is : -999 mBar
21. Pressure sensor absolute is : -999 mBar
22. Sample pump 1: Last calibration of the sample pump was : 01-Jan-0001
23. Alignment check 1 by curvature detector is 1.4816 (1=perfect)
24. Alignment check 2 by curvature detector is 18% (100%=perfect)
25. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached
26. FWS: Nr of saturated signals is 0 (0% of 67450 measured particles)
27. FWS: Maximum signal height found is 4573 mV
28. FWS: Nr of particles with signal height <10 mV is high (14105 part. (21 %))
29. SWS HS: Nr of saturated signals is 0 (0% of 67450 measured particles)
30. SWS HS: Maximum signal height found is 4137 mV
31. SWS HS: Nr of particles with signal height <10 mV is high (56074 part. (83 %))
32. FL Yellow HS: Nr of saturated signals is 0 (0% of 67450 measured particles)
33. FL Yellow HS: Maximum signal height found is 2199 mV
34. FL Yellow HS: Nr of particles with signal height <10 mV is high (62816 part. (93 %))
35. FL Orange HS: Nr of saturated signals is 0 (0% of 67450 measured particles)
36. FL Orange HS: Maximum signal height found is 4026 mV
37. FL Orange HS: Nr of particles with signal height <10 mV is high (49083 part. (73 %))
38. FL Red HS: Nr of saturated signals is 0 (0% of 67450 measured particles)
39. FL Red HS: Maximum signal height found is 4289 mV
40. FL Red HS: Nr of particles with signal height <10 mV is high (25 part. (0 %))
41. FWS: Nr of block shaped signals is 196 (0.29059%)
42. FL Red HS: Nr of block shaped signals is 63 (0.093403%)
43. SWS HS: Nr of block shaped signals is 41 (0.060786%)
44. Suspect nr: 0=low, 10=high
45. Maximum FWS: suspect = 0
46. Maximum SWS HS: suspect = 0
47. Maximum FL Yellow HS: suspect = 1
48. Maximum FL Orange HS: suspect = 1
49. Maximum FL Red HS: suspect = 0
50. SWS HS: PMT level is 0
51. FL Yellow HS: PMT level is 0
52. FL Orange HS: PMT level is 0
53. FL Red HS: PMT level is 0

-------------------------- Warnings info --------------------------
1.05 Warning: Your effective analysis volume is low compared to the pumped analysis volume
1.05.1 Solution - Set blocksize memory in CytoUSB higher, used=4k (default=4k) e.g. to 8k
1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume
1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs)
1.15 Warning: Pressure difference is high.
1.15.1 Solution - Check if there is something blocking from sample inlet to sheath (filter block, tubing)
1.16 Warning: Inner absolute pressure in fluidics towards outside is high.
1.16.1 Solution - Check if there is something blocking in the fluidics pathways (filter block, cuvette, tubing)
1.17 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (01-Jan-0001)
1.17.1 Solution - Check if the pumped analysis volume is the real volume by weighing
1.18 Warning: FWS-L and -R detector signals are quite different (ratio 1.4816(1=perfect)) and might indicate an alignment problem
1.18.1 Solution - Probably you have an air bubble in your system. Pinch valve to get rid of it
1.18.2 Solution - Improve alignment of FWS-L and -R detector towards sample core
1.19 Warning: FWS-L and -R might indicate an air bubble problem 18% (100%=perfect)
1.19.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different
1.23 Warning: SWS HS : Nr of particles with signal height <10 mV is high (83 %)
1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise)
1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample
1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)
1.23 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (93 %)
1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise)
1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample
1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)
1.23 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (73 %)
1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise)
1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample
1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)
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