micro_cend15_19 2020-02-07 16u19.cyz ----- Processed at : 07-Feb-2020 16:29:52 ----- 1. The cytometric analysis is performed on machine Cefas 2. The CytoUSB software used is CytoUSB Version: 5.7.1.0 3. The filename is micro_cend15_19 2020-02-07 16u19.cyz 4. The analysis is performed on 07-02-2020 time 16:19:24 5. The number of measured particles is 31079 6. The total concentration found (e.g. only SWS trigg.) is 16 particles/microliter 7. The concentration found (all used triggers) is 16 particles/microliter 8. The preconcentration (before analysis) found (all used triggers) is 20 particles/microliter 9. The flush time found (between samples) is 180 seconds 10. The effective analysis volume is 1908.5739 microliter 11. The pumped volume is 2472 microliter 12. The particle-rate is 67 particles passing the laser beam/second 13. The used sample flow rate is 4.1168 microliter/second 14. The used block size memory is 4 kB 15. Trigger 1: FL Red at threshold level 18 mV 16. Trigger 2: not used at threshold level -999 mV 17. Temperature sensor PMT is : -999 C 18. Temperature sensor Sheath is : -999 C 19. Temperature sensor Sheath is : 23 C 20. Pressure sensor difference is : -999 mBar 21. Pressure sensor absolute is : -999 mBar 22. Sample pump 1: Last calibration of the sample pump was : 01-Jan-0001 23. Alignment check 1 by curvature detector is 1.7363 (1=perfect) 24. Alignment check 2 by curvature detector is 16% (100%=perfect) 25. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 26. FWS: Nr of saturated signals is 0 (0% of 31079 measured particles) 27. FWS: Maximum signal height found is 3430 mV 28. FWS: Nr of particles with signal height <10 mV is high (4986 part. (16 %)) 29. SWS HS: Nr of saturated signals is 0 (0% of 31079 measured particles) 30. SWS HS: Maximum signal height found is 4026 mV 31. SWS HS: Nr of particles with signal height <10 mV is high (25005 part. (80 %)) 32. FL Yellow HS: Nr of saturated signals is 0 (0% of 31079 measured particles) 33. FL Yellow HS: Maximum signal height found is 785 mV 34. FL Yellow HS: Nr of particles with signal height <10 mV is high (25224 part. (81 %)) 35. FL Orange HS: Nr of saturated signals is 0 (0% of 31079 measured particles) 36. FL Orange HS: Maximum signal height found is 1387 mV 37. FL Orange HS: Nr of particles with signal height <10 mV is high (18966 part. (61 %)) 38. FL Red HS: Nr of saturated signals is 0 (0% of 31079 measured particles) 39. FL Red HS: Maximum signal height found is 4327 mV 40. FL Red HS: Nr of particles with signal height <10 mV is high (262 part. (1 %)) 41. FWS: Nr of block shaped signals is 4 (0.01287%) 42. SWS HS: Nr of block shaped signals is 2 (0.0064352%) 43. FL Red HS: Nr of block shaped signals is 1 (0.0032176%) 44. Suspect nr: 0=low, 10=high 45. Maximum FWS: suspect = 0 46. Maximum SWS HS: suspect = 1 47. Maximum FL Yellow HS: suspect = 2 48. Maximum FL Orange HS: suspect = 1 49. Maximum FL Red HS: suspect = 0 50. SWS HS: PMT level is 0 51. FL Yellow HS: PMT level is 0 52. FL Orange HS: PMT level is 0 53. FL Red HS: PMT level is 0 -------------------------- Warnings info -------------------------- 1.03 Warning: Your preconcentration differs more than 25% from the during analysis concentration 1.03.1 Solution - Carry over between samples? > Flush longer 1.03.2 Solution - Better homogenize samples during subsampling by a gentle stirrer 1.03.3 Solution - Colonies-filaments of cells break during analysis > stir slower 1.09 Warning: This (default) low block size of 4kB in CytoUSB will decrease the effective analysis volume 1.09.1 Solution - Increase block size memory if the total particle rate is high enough (but prevent time outs) 1.15 Warning: Pressure difference is high. 1.15.1 Solution - Check if there is something blocking from sample inlet to sheath (filter block, tubing) 1.16 Warning: Inner absolute pressure in fluidics towards outside is high. 1.16.1 Solution - Check if there is something blocking in the fluidics pathways (filter block, cuvette, tubing) 1.17 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (01-Jan-0001) 1.17.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.18 Warning: FWS-L and -R detector signals are quite different (ratio 1.7363(1=perfect)) and might indicate an alignment problem 1.18.1 Solution - Probably you have an air bubble in your system. Pinch valve to get rid of it 1.18.2 Solution - Improve alignment of FWS-L and -R detector towards sample core 1.19 Warning: FWS-L and -R might indicate an air bubble problem 16% (100%=perfect) 1.19.1 Solution - Pinch valve to get rid of the air bubble or blockage by something different 1.23 Warning: SWS HS : Nr of particles with signal height <10 mV is high (80 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.23 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (81 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.23 Warning: FL Orange HS : Nr of particles with signal height <10 mV is high (61 %) 1.23.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.23.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.23.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls)