EasyClus check & Info of last file ----- Processed at : 23-Apr-2024 18:30:06 ----- 1. The cytometric analysis is performed on machine Lobith 2. The CytoBuoy instrument serial-nr is CS-2021-106 3. The CytoUSB software used is CytoUSB Version: 5.11.3.1 4. LASER : OBIS LS 488-60 : Used at 50 mW 5. LASER : OBIS LS 552-60 : Used at 50 mW 6. The filename is EIJ2_SWS15_FLRed10_auto_uls_IMG_600s 2024-04-23 18h14.cyz 7. The analysis is performed on 23-04-2024 time 18:17:15 8. The number of measured particles is 20115 9. The total concentration found (e.g. only SWS trigg.) is 602 particles/microliter 10. The concentration found (all used triggers) is 11 particles/microliter 11. The preconcentration (before analysis) found (all used triggers) is 11 particles/microliter 12. The flush time found (between samples) is 130 seconds 13. The effective analysis volume is 1836.3378 microliter 14. The pumped volume is 1848 microliter 15. The particle-rate is 1854 particles passing the laser beam/second 16. The used sample flow rate is 3.08 microliter/second 17. Trigger 1: SWS HS at threshold level 15 mV 18. Trigger 2: Maximum FL Red HS at threshold level 10 19. Temperature sensor PMT is : 28.4 C 20. Temperature sensor Sheath is : 23.1 C 21. Temperature sensor Laser is : 28.4 C 22. Temperature sensor System is : 24.8 C 23. Pressure sensor difference is : -601 mBar 24. Pressure sensor absolute is : 457 mBar 25. Sample pump 1: Last calibration of the sample pump was : 01-Jan-1990 26. Alignment check 1 by curvature detector is 0.91024 (1=perfect) 27. Alignment check 2 by curvature detector is 64.2% (>70%=perfect) 28. Saturated signals (flattened peaks) occur when the dynamic range of the (HS)detector is reached 29. FWS: Nr of saturated signals is 1 (0% of 20115 measured particles) 30. FWS: Maximum signal height found is 14464 mV 31. FWS: Nr of particles with signal height <10 mV is high (298 part. (1 %)) 32. SWS HS: Nr of saturated signals is 1 (0% of 20115 measured particles) 33. SWS HS: Maximum signal height found is 7360 mV 34. SWS HS: Nr of particles with signal height <10 mV is high (0 part. (0 %)) 35. FL Yellow HS: Nr of saturated signals is 0 (0% of 20115 measured particles) 36. FL Yellow HS: Maximum signal height found is 2752 mV 37. FL Yellow HS: Nr of particles with signal height <10 mV is high (12204 part. (61 %)) 38. FL Orange HS: Nr of saturated signals is 1 (0% of 20115 measured particles) 39. FL Orange HS: Maximum signal height found is 7360 mV 40. FL Orange HS: Nr of particles with signal height <10 mV is high (3198 part. (16 %)) 41. FL Red HS: Nr of saturated signals is 20 (0% of 20115 measured particles) 42. FL Red HS: Maximum signal height found is 7360 mV 43. FL Red HS: Nr of particles with signal height <10 mV is high (514 part. (3 %)) 44. FL Orange HS: Nr of block shaped signals is 203 (1.0092%) 45. FL Red HS: Nr of block shaped signals is 62 (0.30823%) 46. SWS HS: Nr of block shaped signals is 23 (0.11434%) 47. FWS: Nr of block shaped signals is 21 (0.1044%) 48. FL Yellow HS: Nr of block shaped signals is 15 (0.074571%) 49. Suspect nr: 0=low, 10=high 50. Maximum FWS: suspect = 0 51. Maximum SWS HS: suspect = 0 52. Maximum SWS LS: suspect = 3 53. Maximum FL Yellow HS: suspect = 3 54. Maximum FL Yellow LS: suspect = 0 55. Maximum FL Orange HS: suspect = 3 56. Maximum FL Red HS: suspect = 0 57. Maximum FL Red LS: suspect = 0 58. SWS HS: PMT level is 49 59. SWS LS: PMT level is 48 60. FL Yellow HS: PMT level is 94 61. FL Yellow LS: PMT level is 76 62. Fl Orange: PMT level is 105 63. FL Red HS: PMT level is 110 64. FL Red LS: PMT level is 81 65. IIF: Imaging in Flow pictures available. Nr of images is 1000 -------------------------- Warnings info -------------------------- 1.21 Warning: Last calibration of sample pump towards analysis date was more than 90 days. (01-Jan-1990) 1.21.1 Solution - Check if the pumped analysis volume is the real volume by weighing 1.24 Warning: SWS LS: Maximum is 576 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Yellow LS: HS-LS ratio is 22 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Yellow LS: Maximum is only 7 mV (if HS is around 4500 mV) and too low. 1.24.1 Solution - Put LS PMT higher. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Yellow LS: HS-LS ratio is 32 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.24 Warning: FL Red LS: Maximum is 118 mV if HS is around 4500 mV and too high. 1.24.1 Solution - Put LS PMT lower. Maximum LS around 50 mV (if HS is around 4500 mV). 1.24 Warning: FL Red LS: HS-LS ratio is 62 and too low. 1.24.1 Solution - Put LS PMT lower. Ratio HS/LS should be around 100. 1.27 Warning: FL Yellow HS : Nr of particles with signal height <10 mV is high (61 %) 1.27.1 Solution - Increase, set higher trigger threshold (to prevent measuring noise) 1.27.2 Solution - Decrease, set lower particle sample rate. Maybe caused by an overload of particles/ noise in your sample 1.27.3 Solution - Clean sheath, clean cuvette, clean tubing? (noise can be introduced by dirty walls) 1.25 Warning: Suspected particles data blocks found! 1.29.1: Maximum SWS LS: suspect = 3 1.29.11 Solution - Particle rate too high? 1.29.21 Solution - Trigger too sensitive? 1.29.31 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data' 1.29.2: Maximum FL Yellow HS: suspect = 3 1.29.12 Solution - Particle rate too high? 1.29.22 Solution - Trigger too sensitive? 1.29.32 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data' 1.29.3: Maximum FL Orange HS: suspect = 3 1.29.13 Solution - Particle rate too high? 1.29.23 Solution - Trigger too sensitive? 1.29.33 Solution - Details look at 'DATA DIAGNOSIS' in 'Check CytoSense-Diagnosis-Meta Data'